Catalog Number - 85018-05P

The 85018‑05P Cogent RP C18 ABX™ HPLC Column is engineered for high‑flow, low‑pressure separations under extreme mobile phase conditions, delivering reliable performance across a wide pH range (2–12).

Constructed with a bulky, specially end‑capped C18 phase bonded to high‑purity silica, this column minimizes secondary interactions and improves peak shape for acidic, basic, hydrophobic, and neutral analytes, including compounds with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for demanding acid/base methods

50 mm Length – Fast Method Scouting & Screening

• Designed for rapid separations and short run times

• Ideal for:

  • method development and screening

  • preparative method scouting workflows

• Reduces:

  • solvent usage

  • analysis time

✅ Preferred starting point for scalable chromatography methods

4.6 mm ID – Analytical to Preparative Bridge Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • scale‑up planning

  • analytical validation prior to prep

Typical Flow Rate Range

• ~0.8–1.5 mL/min (higher flows possible due to 10 µm particles)

✅ Serves as a bridge between analytical and preparative chromatography

10 µm Particle – Preparative Scouting & High‑Flow Performance

• Designed for:

  • high flow rates

  • low backpressure operation

  • durability under heavy sample loads

✅ Ideal for:

• Preparative method development and scale‑up workflows

• Systems requiring lower pressure operation

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Operates reliably in:

  • acidic mobile phases

  • basic mobile phases

• Suitable for:

  • strong acids

  • strong bases

✅ Enables separations not achievable with standard C18 phases

Why Choose RP C18 ABX™ Columns (10 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for challenging analytes and aggressive conditions

Preparative Scouting Capability

• Designed for method scalability and transfer to preparative columns

• Enables efficient transition from analytical to prep scale

Low Backpressure Operation

• Supports higher flow rates with reduced system strain

• Compatible with standard HPLC systems

✅ Preferred for method development where scalability is critical

Application Performance Advantages

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

• Enables:

  • Reliable performance in scale‑up workflows

  • Consistent results under extreme pH conditions

✅ Ideal for preparative method scouting and process development

System & Sample Considerations

• Compatible with standard HPLC systems

• Suitable for high‑flow and preparative‑leaning workflows

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain consistent performance

Typical Applications

• Preparative method scouting and scale‑up

• HPLC method development

• Acidic and basic compound separations

• Pharmaceutical process development

• Stability‑indicating methods

• Long‑chain fatty acid analysis

Important Usage Notes

• Designed for standard HPLC systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for method development and preparative scaling workflows

• Optimize flow rates for throughput vs resolution balance